WebAug 8, 2015 · Cell culture. Human monocytic THP-1 cells were maintained in culture in Roswell Park Memorial Institute medium (RPMI 1640, Invitrogen) culture medium containing 10 % of heat inactivated fetal bovine serum (Invitrogen) and supplemented with 10 mM Hepes (Gibco, #15630-056), 1 mM pyruvate (Gibco, #11360-039), 2.5 g/l D-glucose … WebApr 13, 2024 · Exosomes or conditioned media from MDA-MB-231 cells polarize THP1 cells towards the M2 phenotype with the upregulation of CD206 and decreased expression of CD86. However, exosomes from sh-Kaiso cells induced more M1 polarization, whereas THP1 cells showed high expression of the M1 marker CD86 and low expression of the M2 …

Mannose metabolism inhibition sensitizes acute myeloid …

WebTHP-1 cell line (ATCC TIB-202) is commonly used to study monocyte and macrophage activities, functions, innate immune mechanisms and signaling pathways. This luciferase reporter cell line was derived from parental line TIB-202 by stably expressing firefly luciferase gene (luc2) under control of an activator protein 1 (AP1) promoter. This cell line … WebJan 12, 2024 · It takes a minute or two longer, but it’s worth it. Make sure you use enough freezing medium for your cells or the cells will not survive the tundra. Once the cells are suspended, seal the tube, invert a few times, … hemlock varnish mushroom

THP1-Dual NF-κB & IFN Reporter THP1 monocyte InvivoGen

WebThe tumor-derived conditioned medium decreased the proliferation of THP-1 cells and blocked their cell cycle at the G1 stage. The tumor-conditioned medium also upregulated the production of several cytokines and chemokines characteristic of both M1 and M2 subsets and induced the expression of important pro-angiogenic factors, vascular endothelial … WebThe formulation is based on RPMI-1630 medium and uses a bicarbonate buffering system and has alterations in the amounts of amino acids and vitamins. When RPMI is properly supplemented, it has a wide applicability for supporting growth of many types of cell cultures including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. WebRemove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep … hemlock versus cedar mulch