Phospho antibody
WebThe NeuroMab™ anti-Phospho-p53 (Ser15) antibody (Rodent Specific) recognizes endogenous levels of p53 protein only when phosphorylated at Ser15. Expression Host Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser15 of mouse p53 protein. Species Reactivity … WebPhosphospecific antibodies are affinity-purified rabbit polyclonal or monoclonal antibodies that are monospecific for a target protein that is phosphorylated. These antibodies provide critical insight into the investigation of complex signal transductions events. On this page … Explore our diverse products for Immunoassays including matched … Antibody fragmentation is somewhat laborious, requires optimization of … Recombinant rabbit monoclonal antibodies are developed by immunizing animals … Antibody screening, titering and isotyping are important in-process and final …
Phospho antibody
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WebAll synaptic proteins contain a short amino-terminal domain, which is highly conserved and phosphorylated by PKA or CaM kinase i. The phosphorylation of the amino terminal serine … WebPhospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb recognizes endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. This …
WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived … WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization , Foxp3/Transcription Factor Buffer, and IC …
WebTo use the phospho-specific antibodies as an assay for Raf-1 activation, the Raf-1 protein is first immunoprecipitated from cell lysates with an antibody that efficiently immunoprecipitates Raf-1. The immunoprecipitates are then washed, separated by SDS–PAGE, and transferred onto a 0.2-μm pore size nitrocellulose membrane as … WebAnti-PD1 (phospho Y248) antibody [EPR19821] See all PD1 primary antibodies Description Rabbit monoclonal [EPR19821] to PD1 (phospho Y248) Host species Rabbit Tested applications Suitable for: WB, Dot blot, …
WebAnti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample Other Materials Required Distilled or deionized water 100 ml and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors
WebPhospho-S6 (Ser235, Ser236) Antibody (MA5-15140) in ICC/IF. Immunofluorescence analysis of Phospho-S6 (Ser235,Ser236) was performed using 70% confluent log phase U … data specification in software engineeringWebAnti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244) from rabbit, purified by affinity chromatography Synonym (s): 40S ribosomal protein S6, Phosphoprotein NP33 eCl@ss: 32160702 Pricing and availability is not … data specification sheetWebThe Phospho- (Ser/Thr) Kinase Substrate Antibody Sampler Kit provides an economical means to investigate the downstream activity of select serine/threonine kinases. The kit … dataspeed githubWebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. data speed in windows 10WebCheck the product webpage/datasheet for recommended antibody dilution. Note that while the membrane is blocked with nonfat dry milk, phospho-specific antibodies are often diluted and incubated with the membrane in 5% BSA. Use TBS-T rather than PBS-T in wash/incubation buffers since the phosphate ion may lead to interference.* data speed meter for windows 10 downloadWebImmunofluorescent analysis of Phospho-PPAR alpha pSer12 (green) showing staining in the nucleus of 3T3-L1 cells (right) compared to a negative control without primary antibody … bitter lowest thresholdbitterly clinging to guns and religion