WebA 116 base-pair PCR product derived from a vWA locus was amplified by Taq, Pfu, or KOD DNA polymerase and compared by agarose gel electrophoresis and electrospray … WebThe system includes an ultra high fidelity KOD DNA polymerase complexed with two monoclonal antibodies to permit hot start thermocycling, along with specially formulated 2X buffer. KOD Xtreme Hot Start DNA Polymerase quickly and accurately amplifies genomic and phage/plasmid DNA targets up to 24 and 40 kbp, respectively.

Identification and functional analysis of glycosyltransferase ...

WebApr 15, 2011 · We used high success-rate DNA polymerase KOD FX to circumvent these PCR amplification problems. ... (25 mmol/L) 1.5 µL, dNTPs (10 mmol/L) 2.0 µL, primer (10 pmo1/µL) 1 µL, Taq polymerase (5 U/µL) 0.2 µL, and genomic DNA (50 ng/µL) 1 µL. The PCR parameters were 94°C for 5 min, 38 cycles of 94°C for 1 min, 38°C for 1 min, and 72°C for ... WebJun 27, 2016 · The high efficient and success rate DNA polymerase KOD FX Neo (TOYOBO, CO.,LTD) was used for confirmation by colony PCR. The products were sequenced as needed by Sangon Biotech (Shanghai) Co., Ltd. The TRP1-ORF-YZ-F/TRP1-YZ-R primer set was used for confirmation by PCR for the TRP1 experiments. north america human trafficking conference

High efficient & High fidelity PCR enzyme KOD FX

WebIn this article, development of PCR enzymes including (1) Characterization of a new PCR enzyme, KOD DNA polymerase, (2) Engineering of a new long and accurate (LA) PCR … WebNEW: Q5U ® Hot Start High-Fidelity DNA Polymerase is a modified version of Q5 High-Fidelity DNA Polymerase containing a mutation in the uracil-binding pocket that enables … WebM1：1kb DNA Ladder 1：KOD FX 2：KOD -Plus-3：KOD -Plus-+5% DMSO 4：A公司酶 5：B公司酶 6：C公司酶 7：Taq DNA Pol. M2：λ/ Hind III digest 人TGF-β2.3kb (GC含量约70%) M112 34 567M2 123 4 56 M2 人IGF2R基因[NM_000876] 8.9kb (GC含量约90%区域的mRNA) M1：1kb DNA Ladder 1：KOD FX 2：A公司酶 3: D公司酶 4: E公司 ... north america hour