Hcs lipidtox red neutral lipid stain
WebTransmembrane protein 68 (TMEM68) is an endoplasmic reticulum (ER)-anchored acyltransferase family member of unknown function. In the current study we show that overexpression of TMEM68 promotes TG accumulation and lipid droplet (LD) formation in a conserved active sites-dependent manner. WebHCS LipidTOX™ Red neutral lipid stain 125 µL Solution in DMSO (1000X for steatosis assay; 200X for adipogenesis assay <–20°C Desiccate Protect from light Avoid …
Hcs lipidtox red neutral lipid stain
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Web3.1 Prepare LipidTOX™ Green neutral lipid stain. Dilute the 1000X LipidTOX™ Green neutral lipid stain 1:1000 in buffer to make a 1X solution. A volume of 100 µL/well is required. 3.2 Remove buffer from the cells (after the appropriate number of buffer washes from step 2.6). 3.3 Add 100 µL of 1X LipidTOX™ Green neutral lipid stain to ... WebJan 1, 2014 · HCS LipidTOX Red staining 1. LipidTOX Red staining solution: Dilute HCS LipidTOX Red (Invitrogen) 1:200 in PBS. Prepare 40 μl per cover slip; allow 10% extra for pipetting errors. 2. 0.2% Triton X-100 in PBS (v/v). 3. Incubation chamber: Prepare a dark chamber big enough to store all cover slips.
WebJul 11, 2014 · Immunofluorescence and HCS LipidTox Red neutral lipid staining. Cells grown on coverslip or in labtec II chamber slides (Nunc) were fixed in 4% paraformaldehyde for 1 h at room temperature, washed with PBS, and permeabilized in 0.1% Triton X-100 for 5 min or 0.5% saponin for 5 min. After incubation in blocking solution (5% BSA, 1% fish … Web(HCS LipidTOX™ Deep Red Neutral Lipid Stain) for lipid imaging. Upon excitation at 561 nm, numerous red uorescent spots were observed; again, these were found to colocalize with commercial dye (LysoTracker® Blue DND-22) for lysosome imaging (Fig. 1, second panel). However, these two uorescent
WebJan 29, 2024 · Cells were cultured, stained with Hoechst 33258 and HCS LipidTOX™ Red Neutral Lipid Stain, and then observed under the confocal microscope system, Leica TCS SP8. a Three-dimensional reconstitution of cells expressing BiP-EGFP (2-day culture). The image was created from seven optical sections taken at 0.8-μm intervals along the … WebLipidTox stains live cells nicely, and the lipid droplets are stable for a few days as long as the cells are healthy. There will be basal uptake of the stain into non-lipid droplet containing ...
WebJun 8, 2024 · Alternatives: An alternative to using BODIPY 558/568 C 12 to label LDs is to employ a neutral lipid stain such as 10 μg/mL BODIPY 493/503. 15. ... HCS …
WebCatalog number: H34475. The intracellular accumulation of neutral lipids, steatosis, is often triggered by drugs that affect the metabolism of fatty acids and/or neutral lipids. HCS … lawn mower utility tiltdeck trailerWebNov 9, 2024 · Chloroquine increased the intensity of HCS LipidTOX deep red neutral lipid staining in J774, RAW264.7, THP-1, and M2 macrophages and area in DMBM-2 and … kaniche reserve rumWebDec 14, 2009 · Neutral lipid droplets and phospholipidosis were analyzed using Invitrogen's HCS LipidTOX™ Phospholipidosis and Steatosis Detection Kit for image-based high-content screening assays. Staining … kanibal and company jersey city njWebHcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … lawn mower using too much gasWebA collection of spectra representing the microscope Hoffman Lab Olympus IX83 on FPbase. lawn mower utility shedWebJan 13, 2024 · Then, cells were stained with HCS LipidTOX Red neutral lipid stain (H34476, Thermo Fisher Scientific) for 30 min. Nuclei were stained with DAPI for 5 min. All slides were mounted with VECTASHIELD ... lawn mower utility cartsWebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is … kanicho2 gmail.com