WebEscherichia coli cytolysin A (ClyA) is an α-helical pore-forming toxin (PFT) which lyses target cells by forming membrane permeabilizing pores. The rate-determining step of this process is the conversion of the soluble ClyA monomer into a membrane inserted protomer. We elucidate the mechanism of this conformational transition using molecular dynamics … WebThe cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice. Vibrio vulnificus, a gram-negative estuarine bacterium, causes wound infections and septicemia in humans, mostly in immunocompromised people and those with underlying conditions such as hemochromatosis, ...
2WCD: Crystal structure of the assembled cytolysin A pore - RCSB
WebCertain strains of Escherichia coli, Salmonella enterica and Shigella flexneri produce a pore-forming toxin hemolysin E (HlyE), also known as cytolysin A (ClyA) and silent hemolysin, locus A (SheA). HlyE lyses erythrocytes and mammalian cells, forming transmembrane pores with a minimum internal diameter of ∼25 Å. We review the current knowledge of … WebBy leveraging the ability of bacteria to express therapeutic protein cytolysin A (ClyA) through plasmid transformation, a thermally-activated biohybrid (TAB@Au) is … howden vacancies south africa
[PDF] Conformational flexibility is a key determinant of the lytic ...
WebBy leveraging the ability of bacteria to express therapeutic protein cytolysin A (ClyA) through plasmid transformation, a thermally-activated biohybrid (TAB@Au) is constructed by biomineralizing gold nanoparticles (AuNPs) on the E. coli surface. Due to the feature of anaerobic bacteria homing to tumor microenvironments, the bacteria-based antitumor … WebThe Vibrio cholerae cytolysin (VCC), formerly known as hemolysin, a very efficiently synthesized PFT, turned out to be a conserved protein efficiently exported by these Gram-negative bacteria. The monomeric VCC is released as a 65 kDa water soluble mature protein; depending on the strain, its production may be very efficient, and in some ... WebGroup B streptococci (GBS) express a beta-haemolysin/cytolysin that contributes to disease pathogenesis. We report an independent discovery and extension of a genetic locus encoding the GBS beta-haem howden uk limited