WebSep 1, 2024 · 配制10%的分离胶和5% 的浓缩胶,每孔加50 μg蛋白上样,进行SDS-PAGE后电转印到PVDF膜,5%脱脂奶粉封闭2 h,加入1∶1 000稀释 的p-PI3K、PI3K、p-Akt、Akt、p-FoxO1、FoxO1、GLUT-2和β-Actin一抗,4 ℃孵育14 h,TBST漂洗后加1∶2 000稀释的HRP-IgG二抗,室温孵育2 h,TBST充分漂洗后加 ... WebApr 9, 2024 · Furthermore, FoxO1, on Klotho/FoxO1 signalling pathway, was increased in the parishin-intervened group, accompanied by the downregulated phosphorylated FoxO1. ... ,Phospho-FoxO1 (Ser256) (CST #9461), IL-6 (ab 6672, Abcam), γH2AX (ab 11174), PARP-1 (CST #9532), p16INK4a (ab 81278), peroxisome proliferator-activated receptor …
FoxO1 (C29H4) Rabbit mAb from Cell Signaling Technology
WebMar 9, 2005 · In the present study, we show that FOXO1 interacts with four and a half LIM 2 (FHL2) in prostate cancer cells. This interaction occurred in the nucleus and was enhanced by lysophosphatic acid. FHL2 decreased the transcriptional activity of FOXO1 and the expression of known FOXO target genes and inhibited FOXO1-induced apoptosis. WebForkhead box O (FOXO) proteins are a family of transcription factors with four members in mammals, namely FOXO1, FOXO3a, FOXO4, and FOXO6. FOXO factors, originally … folgers coffee water ratio
miR-378-mediated glycolytic metabolism enriches the Pax7Hi ...
WebNov 1, 2011 · The forkhead box O (FoxO) transcription factor family is a key player in an evolutionary conserved pathway downstream of insulin and insulin-like growth factor receptors. The mammalian FoxO family consists of FoxO1, 3, 4 and 6, which share high similarity in their structure, function and regulation. FoxO proteins are involved in diverse ... WebThis study investigated the role of miR-3188 on the proliferation of non-small cell lung cancer cells and its relationship to FOXO1-modulated feedback loop. Two non-small cell lung cancer (NSCLC) cell lines A549 and H1299 were used. RNA silencing was achieved by lentiviral transfection. Cell proliferation was assessed by immunohistochemical staining … WebNov 2, 2024 · The FoxO transcription factor family belongs to the Forkhead box transcription factors consisting of DAF-16 in Caenorhabditis elegans, dFOXO in Drosophila Melanogaster, and four members in mammals: FoxO1, FoxO3, FoxO4 and FoxO6.FoxO proteins receive and integrate various upstream signals and, through post-translational … ehc winterthur fanshop